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RESEARCH PRODUCT
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.
Eitan LernerAnders BarthJelle HendrixBenjamin AmbroseVictoria BirkedalScott C. BlanchardRichard BörnerHoi Sung ChungThorben CordesTimothy D. CraggsAshok A. DenizJiajia DiaoJingyi FeiRuben L. GonzalezIrina V. GopichTaekjip HaChristian A. HankeGilad HaranNikos S. HatzakisSungchul HohngSeok Cheol HongThorsten HugelAntonino IngargiolaChirlmin JooAchillefs N. KapanidisHarold D. KimTed LaurenceNam Ki LeeTae Hee LeeEdward A. LemkeEmmanuel MargeatJens MichaelisXavier MichaletSua MyongDaniel NettelsThomas Otavio PeulenEvelyn PloetzYair RazvagNicole C. RobbBenjamin SchulerHamid SoleimaninejadChun TangReza VafabakhshDon C. LambClaus A.m. SeidelShimon WeissOlga Boudkersubject
0301 basic medicineconformationOpen scienceComputer scienceStructural Biology and Molecular BiophysicsAMINOACYL-TRANSFER-RNAINTRAMOLECULAR DISTANCE DISTRIBUTIONSReview ArticleRESONANCE ENERGY-TRANSFER01 natural sciencesbiomoleculesFREELY DIFFUSING MOLECULESDocumentationFluorescence Resonance Energy TransferMainstreamstructural biologyBiology (General)General NeuroscienceQRNANO-POSITIONING SYSTEMGeneral MedicinedynamicsINTRINSICALLY DISORDERED PROTEINSSingle Molecule ImagingFLUORESCENCE CORRELATION SPECTROSCOPY[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/BiophysicsMedicinecommunitysingle-moleculeQH301-705.5ScienceAppeal[SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry Molecular Biology/BiophysicsBioengineeringchemical biology010402 general chemistryGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesALTERNATING-LASER EXCITATIONBiochemistry and Chemical Biologymolecular biophysicsbiochemistryMolecular BiologyStructure (mathematical logic)General Immunology and MicrobiologySINGLE-MOLECULE FRETTRANSITION PATH TIMESData science0104 chemical sciences030104 developmental biologyFRETPosition paperGeneric health relevanceBiochemistry and Cell Biologydescription
International audience; Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever- increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using various procedures. These multi-lab studies have led to the development of smFRET procedures and documentation, which are important when submitting entries into the archiving system for integrative structure models, PDB- Dev. This position paper describes the current ‘state of the art’ from different perspectives, points to unresolved methodological issues for quantitative structural studies, provides a set of ‘soft recommendations’ about which an emerging consensus exists, and lists openly available resources for newcomers and seasoned practitioners. To make further progress, we strongly encourage‘open science’ practices.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-03-29 | eLife |