6533b838fe1ef96bd12a3ef2
RESEARCH PRODUCT
Monitoring complex bacterial communities using culture-independent molecular techniques: application to soil environment
Lionel RanjardFranck PolySylvie Nazaretsubject
DNA BacterialGenetics0303 health sciencesBacteria030306 microbiology[SDV]Life Sciences [q-bio]Population structureGeneral MedicineBIOLOGIE MOLECULAIREBiologyIsolation (microbiology)Microbiology03 medical and health sciences[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyMicrobial population biologyMicrobial ecologyBiochemical engineering[SDV.MP] Life Sciences [q-bio]/Microbiology and ParasitologyMolecular BiologyCulture independentSoil microbiologyEcosystemSoil MicrobiologyComputingMilieux_MISCELLANEOUS030304 developmental biologydescription
Over the last decade, important advances in molecular biology led to the development of culture-independent approaches to describing bacterial communities. These new strategies, based on the analysis of DNA directly extracted from environmental samples, circumvent the steps of isolation and culturing of bacteria, which are known for their selectivity leading to a non-representative view of the extent of bacterial diversity. This review provides an overview of the potentials and limitations of some molecular approaches currently used in microbial ecology. Examples of applications to the study of indigenous soil microbial community illustrate the feasibility and the power of such approaches.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-01-01 |