6533b838fe1ef96bd12a488c
RESEARCH PRODUCT
Identification of the mstE Gene Encoding a Glucose-inducible, Low Affinity Glucose Transporter in Aspergillus nidulans
Andrew MaccabeDaniel RamónDaniel RamónLuisa VenturaJosep V. FormentJosep V. FormentMichel Flipphisubject
DNA ComplementaryDatabases FactualMonosaccharide Transport ProteinsRecombinant Fusion ProteinsGlucose uptakeGenes FungalGreen Fluorescent ProteinsMolecular Sequence DataHyphaeRepressorBiochemistryAspergillus nidulansSubstrate SpecificityFungal ProteinsCell membraneAspergillus nidulansGene Expression Regulation FungalmedicineAmino Acid SequenceMolecular BiologyGenePhylogenyExpressed Sequence TagsFungal proteinbiologyCell MembranefungiGlucose transporterCell BiologySpores FungalBlotting Northernbiology.organism_classificationFusion proteinRepressor ProteinsKineticsGlucosemedicine.anatomical_structureMicroscopy FluorescenceBiochemistryGene Deletiondescription
The mstE gene encoding a low affinity glucose transporter active during the germination of Aspergillus nidulans conidia on glucose medium has been identified. mstE expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor CreA. The expression of MstE and its subcellular distribution have been studied using a MstE-sGFP fusion protein. Concordant with data on mstE expression, MstE-sGFP is synthesized in the presence of repressing carbon sources, and fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane. Deletion of mstE has no morphological phenotype but results in the absence of low affinity glucose uptake kinetics, the latter being substituted by a high affinity system.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-01-19 | Journal of Biological Chemistry |