6533b838fe1ef96bd12a50c9
RESEARCH PRODUCT
Genomic response programs of Saccharomyces cerevisiae following protoplasting and regeneration.
Eulogio ValentínJosé Ruiz-herreraRafael SentandreuLuis CastilloSamuel GelisAna I. Martínezsubject
GeneticsSaccharomyces cerevisiae ProteinsbiologyReverse Transcriptase Polymerase Chain ReactionGene Expression ProfilingProtoplastsSaccharomyces cerevisiaeGenomicsSaccharomyces cerevisiaeProtoplastbiology.organism_classificationMicrobiologyCell biologyGene expression profilingTranscription (biology)Cell WallGene Expression Regulation FungalGene expressionGeneticsDNA microarrayCandida albicansGeneOligonucleotide Array Sequence Analysisdescription
Abstract Global transcription profiling during regeneration of Saccharomyces cerevisiae protoplasts was explored. DNA microarrays measured the expression of 6388 genes and wall removal resulted initially in over-expression of 861 genes that decayed later on, a behaviour expected from a transient stress response. Kinetics of expression divided the genes into 25 clusters. Transcription of the genes from clusters 14–25 was initially up-regulated, suggesting that the grouped genes permitted cell adaptation to the removal of the wall. Clustering of genes involved in “wall structure and biosynthesis” showed that most of them had initially low levels of expression that increased along the process. Analysis by use of the T-profiler showed that the group of “structural components of the wall” was up-regulated after two hours and remained as such during the process. These results evoke the likeness and difference with the process occurring in Candida albicans.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-04-23 | Fungal genetics and biology : FGB |