6533b838fe1ef96bd12a51df
RESEARCH PRODUCT
Improved Solid Phase Extraction Procedure for Assay of Cephalosporins in Human Urine Samples
Rosa Herráez-hernándezAdela Sevillano-cabezaLuisa Gallo MartinezPilar Campí Ns-falcósubject
Detection limitChromatographyChemistryClinical BiochemistryExtraction (chemistry)Pharmaceutical ScienceUrineBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryCefalexinmedicineSolid phase extractionCefuroximeAntibacterial agentmedicine.drugdescription
Abstract Solid phase extraction technique has been evaluated for the treatment of urine samples in the analysis of cephalosporins before injection into an HP-Hypersil ODS-C18 column. Cephalexin, cefotaxime, cefazolin, cefuroxime, and cefoxitin were tested with seven different reversed-phase extraction column cartridges and the obtained urine extracts were not clean. However, 3M Empore extraction disk cartridges packed with octadecyl (C18) bonded silica provided clean extracts with a single extraction. The recoveries of the five cephalosporins ranged from 56 to 60 % in the 1.25–500 g/mL concentration range. The assay was accurate, precise, and adequate for testing the drug content in urine samples. The detection limit found for the five drugs was 0.25 g/mL after an injection of 10 L. The reliability of this study was tested by analyzing a urine sample containing cefuroxime after a minimum single dose administration (250 mg).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-08-01 | Journal of Liquid Chromatography & Related Technologies |