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RESEARCH PRODUCT

Der Einfluss des RANTES −403 Genpolymorphismus auf den Langzeitverlauf nach orthotoper Lebertransplantation

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Introduction: By the recruitment of leukocytes chemokines are able to mediate a protective as well as a harming influence on rejections after human orthotopic liver transplantation. There is a base exchange (G/A) known at position-403 in the RANTES promoter region which leads to an increased RANTES expression. Due to the crucial influence of a raised hepatic RANTES synthesis on the course after liver transplantation we investigated the effect of the RANTES −403 polymorphism on organ survival and rejection frequency. Materials and methods: Genomic DNA has been extracted from EDTA-blood of liver transplanted patients. The RANTES −403 polymorphism has been analysed by hybridisation with FRET (fluorescence resonance energy transfer) probes (LightCycler). Results: n = 192 patients (66% male; 34% female) transplanted between 09/1997 and 03/2004 have been tested. The patients were aged 16 to 69 (median: 53). 124 patients (64%) have been homozygous for the G-allele of the RANTES −403 polymorphism (G/G), 57 patients (30%) carried the G/A and 11 patients (6%) the A/A alleles.