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RESEARCH PRODUCT
Effects of a phycotoxin, okadaic acid, on oyster heart cell survival
Hélène TalarminGermaine DorangeMickaël DroguetJean-pierre PennecWerner E.g. MüllerHeinz-christoph SchröderMaxime Giouxsubject
ChronotropicOysterbiologyKinaseHealth Toxicology and MutagenesisPhosphataseProtein phosphatase 2Okadaic acidPollutionMolecular biologychemistry.chemical_compoundchemistrybiology.animalEnvironmental ChemistryDiarrhetic shellfish poisoningProtein kinase Cdescription
Okadaic acid (OA) is a dinoflagellate toxin which accumulates in shellfish producing diarrhetic shellfish poisoning (DSP) in humans. It was found that OA is a highly selective inhibitor of protein phosphatase types 1 (PP1) and 2A (PP2A) which produces a marked increase in phosphorylation of several proteins, including p38 mitogen-activated protein (MAP) kinase. The cytotoxicity attributed to OA and the effects on p38 MAP kinase and calcium current were examined in the oyster Crassostrea gigas in this study. Data showed that p38 MAP kinase is strongly expressed in oyster heart and that OA bioaccumulated in cultured heart cells. Hence the effects of OA was tested in vitro and in vivo on oysters. OA was found to (i) exert a positive chronotropic effect on cultured atrial cardiomyocytes which is related to an increase in calcium current via PKC as shown by patch clamp measurements, (ii) produce an activation/phosphorylation of MAP kinase as shown by Westernblot while the non-phosphorylated p38 remained consta...
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-01-01 | Toxicological & Environmental Chemistry |