6533b839fe1ef96bd12a5a9e

RESEARCH PRODUCT

The Conserved Foot Domain of RNA Pol II Associates with Proteins Involved in Transcriptional Initiation and/or Early Elongation

M. Carmen Mirón-garcíaM. Carmen García-lópezAna I. Garrido-godinoMichel WernerOlga CalvoFrancisco NavarroVicente PelechanoVicente PelechanoJosé E. Pérez-ortínAlicia García

subject

GeneticsGuanylyltransferaseChromatin ImmunoprecipitationSequence Homology Amino AcidTranscription GeneticMolecular Sequence DataRNA polymerase IISaccharomyces cerevisiaeInvestigationsBiologyReal-Time Polymerase Chain Reactionchemistry.chemical_compoundchemistryTranscription (biology)Capping enzymeRNA polymeraseGeneticsTranscriptional regulationbiology.proteinAmino Acid SequenceRNA Polymerase IITranscription factor II DGeneConserved Sequence

description

RNA polymerase (pol) II establishes many protein-protein interactions with transcriptional regulators to coordinate different steps of transcription. Although some of these interactions have been well described, little is known about the existence of RNA pol II regions involved in contact with transcriptional regulators. We hypothesize that conserved regions on the surface of RNA pol II contact transcriptional regulators. We identified such an RNA pol II conserved region that includes the majority of the >foot> domain and identified interactions of this region with Mvp1, a protein required for sorting proteins to the vacuole, and Spo14, a phospholipase D. Deletion of MVP1 and SPO14 affects the transcription of their target genes and increases phosphorylation of Ser5 in the carboxyterminal domain (CTD). Genetic, phenotypic, and functional analyses point to a role for these proteins in transcriptional initiation and/ or early elongation, consistent with their genetic interactions with CEG1, a guanylyltransferase subunit of the Saccharomyces cerevisiae capping enzyme. © 2011 by the Genetics Society of America.

yearjournalcountryeditionlanguage
2011-01-01Genetics
https://doi.org/10.1534/genetics.111.133215