6533b839fe1ef96bd12a5aec

RESEARCH PRODUCT

On the zopiclone enantioselective binding to human albumin and plasma proteins. An electrokinetic chromatography approach

M.j. Medina-hernándezYolanda Martín-bioscaSalvador SagradoSalvador SagradoLucía Asensi-bernardi

subject

Plasma protein bindingBiochemistryPiperazinesAnalytical ChemistryCapillary electrophoresisAlbuminsmedicineHumansLeast-Squares AnalysisChromatography Micellar Electrokinetic Capillarychemistry.chemical_classificationChromatographyCyclodextrinOrganic ChemistryEnantioselective synthesisReproducibility of ResultsStereoisomerismBlood ProteinsGeneral MedicineHuman serum albuminBlood proteinschemistryStability constants of complexesEnantiomerAzabicyclo CompoundsProtein Bindingmedicine.drug

description

In this work, a methodology for the chiral separation of zopiclone (ZPC) by electrokinetic chromatography (EKC) using carboxymethylated-β-cyclodextrin as chiral selector has been developed and applied to the evaluation of the enantioselective binding of ZPC enantiomers to HSA and total plasma proteins. Two mathematical approaches were used to estimate protein binding (PB), affinity constants (K(1)) and enantioselectivity (ES) for both enantiomers of ZPC. Contradictory results in the literature, mainly related to plasma protein binding reported data, suggest that this is an unresolved matter and that more information is needed. Discrepancies and coincidences with previous data are highlighted.

yearjournalcountryeditionlanguage
2011-05-01Journal of Chromatography A
https://doi.org/10.1016/j.chroma.2011.03.025