6533b839fe1ef96bd12a5b28

RESEARCH PRODUCT

Differences in the temperature dependencies of uptake of botulinum and tetanus toxins in Aplysia neurons

Anton De PaivaBernard PoulainUlrich WellerJ. Oliver DollyLadislav Tauc

subject

NeuronsBotulinum ToxinsGeneral Neurosciencemedia_common.quotation_subjectTemperatureBiologybiology.organism_classificationAcetylcholineSynapseTetanus ToxinAplysiaAplysiamedicineExtracellularBiophysicsAnimalsNeurotoxinCholinergicInternalizationNeuroscienceAcetylcholineIntracellularmedia_commonmedicine.drug

description

The respective neuroselective actions of botulinum type A (BoNT) and tetanus (TeTx) neurotoxins on cholinergic and non-cholinergic synapses of Aplysia are mainly due to differences in their extracellular neuronal targetting. Further information was gained on this neuroselectivity by examining the temperature dependencies of binding, internalization and intracellular action of both toxins. After reduction of temperature from 22 degrees C to 10 degrees C, the binding of neither BoNT nor TeTx was significantly altered whereas the neuronal uptake of BoNT, but not of TeTx, was prevented. Although TeTx internalization could be detected at the low temperature, its intracellular activity was greatly attenuated compared to that of BoNT. It is inferred that the uptake mechanisms are different for these two related but distinct toxins.

yearjournalcountryeditionlanguage
1992-05-01Neuroscience Letters
https://doi.org/10.1016/0304-3940(92)90573-p