6533b839fe1ef96bd12a6568
RESEARCH PRODUCT
Simple and sensitive reversed-phase liquid chromatographic assay for analysis of chlorthalidone in urine
Pilar Campí Ns-falcóRosa Herráez-hernándezA. Sevtllano-cabezasubject
Detection limitTriamtereneChromatographyChemistryExtraction (chemistry)medicineMolecular MedicineChlorthalidoneSolid phase extractionReversed-phase chromatographyHigh-performance liquid chromatographyQuantitative analysis (chemistry)medicine.drugdescription
Abstract This study describes a rapid method for the determination of chlorthalidone in human urine by reversed-phase liquid chromatography and UV detection at 230 nm, after clean-up over a C8 solid-phase extraction column. Liquid chromatography was carried out on a C18-bonded phase using acetonitrile-phosphate buffer (pH=3) gradient elution. Triamterene was used as internal standard. The system has been applied to the determination of chlorthalidone in the 0.10–10.0 μg/mL concentration interval; the limit of detection was 6 ng/mL.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-08-01 |