6533b839fe1ef96bd12a66c9
RESEARCH PRODUCT
Evolution of the leucine gene cluster in Buchnera aphidicola: insights from chromosomal versions of the cluster.
Roeland C. H. J. Van HamFrancisco J. SilvaAmparo LatorreBeatriz Sabater-muñozAndrés Moyasubject
ChaitophorinaeSubfamilygenome sequenceGenetics and Molecular BiologyMicrobiologyGenomemolecular characterizationsymbiotic bacteriaPlasmidschizaphis-graminumBuchneraLeucinemitochondrial-dnaplasmidGene clusterMolecular BiologyGeneHeat-Shock ProteinsPhylogenyGeneticsRecombination GeneticBinding SitesbiologyEscherichia coli ProteinsChromosomeChromosomes Bacterialbiology.organism_classificationPRI Bioscienceaphidsendosymbiotic bacteriaMultigene Familyescherichia-coliBuchneraanthranilate synthase trpegPlasmidsdescription
ABSTRACT In Buchnera aphidicola strains associated with the aphid subfamilies Thelaxinae, Lachninae, Pterocommatinae, and Aphidinae, the four leucine genes ( leuA , - B , - C , and - D ) are located on a plasmid. However, these genes are located on the main chromosome in B. aphidicola strains associated with the subfamilies Pemphiginae and Chaitophorinae. The sequence of the chromosomal fragment containing the leucine cluster and flanking genes has different positions in the chromosome in B. aphidicola strains associated with three tribes of the subfamily Pemphiginae and one tribe of the subfamily Chaitophorinae. Due to the extreme gene order conservation of the B. aphidicola genomes, the variability in the position of the leucine cluster in the chromosome may be interpreted as resulting from independent insertions from an ancestral plasmid-borne leucine gene. These findings do not support a chromosomal origin for the leucine genes in the ancestral B. aphidicola and do support a back transfer evolutionary scenario from a plasmid to the main chromosome.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-01-01 | Journal of bacteriology |