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RESEARCH PRODUCT
Chemistry of Enzyme Visualization
Gunter M. Rothesubject
chemistry.chemical_classificationchemistry.chemical_compoundEnzymePrimary reactiongenetic structuresChemistrySubstrate specificitySubstrate (chemistry)FormazanCombinatorial chemistryReaction productVisualizationCatalysisdescription
The basic principle of enzyme visualization in situ is to present an enzyme with a solution containing an enzyme specific substrate. Demonstration of an enzyme is achieved if the catalytic action of the enzyme on this substrate produces a coloured reaction product. Often, however, the primary reaction products are colourless and require coupling with a visualizing agent to generate a coloured, preferably insoluble, final reaction product.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-01-01 |