6533b83afe1ef96bd12a712a
RESEARCH PRODUCT
Base composition of DNA from glomalean fungi: high amounts of methylated cytosine.
Michel HosnyVivienne Gianinazzi-pearsonHubert DulieuJean-paul Pais De Barrossubject
Hot Temperature[SDV.GEN] Life Sciences [q-bio]/GeneticsBiologyNucleic Acid DenaturationMicrobiology030308 mycology & parasitology03 medical and health scienceschemistry.chemical_compoundNucleic acid thermodynamicsCytosineBotanyGeneticsDNA FungalChromatography High Pressure Liquid030304 developmental biology[SDV.GEN]Life Sciences [q-bio]/Genetics0303 health sciencesBase CompositionfungiFungiDNA MethylationSpores Fungalbiology.organism_classificationSporeNuclear DNAArbuscular mycorrhiza5-Methylcytosinechemistry5-MethylcytosineCytosineDNAGC-contentdescription
Glomales (Zygomycetes) are obligate fungal symbionts of roots of land plants and form arbuscular mycorrhiza. Sporal DNA of 10 isolates belonging to nine species was purified and the base composition was determined by RP-HPLC. Base composition fell in a narrow range between 30 and 35% G + C. A high amount of methylated cytosine (mC) accounting for 2-4% of the total nucleotides was found in all taxa. The DNA melting profile was defined for Scutellospora castanea. It corresponded to 32% G + C, and the shape of the denaturation curve suggested a heterogeneity in the GC content within the fungal genome. Knowledge of GC contents and variations between taxa are essential for evaluating nuclear DNA content using fluorimetric methods, and high proportions of mC/C + mC in the genomes of glomalean fungi could reflect the existence of repeated DNA families. Results are discussed in relation to data for other fungi and eukaryotes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-01-01 | Fungal genetics and biology : FGB |