6533b83afe1ef96bd12a71ea
RESEARCH PRODUCT
Drastic Genome Reduction in an Herbivore's Pectinolytic Symbiont.
Eugen BauerHassan SalemHassan SalemMartin KaltenpothMartin KaltenpothTakema FukatsuRyuichi KogaMichael G. CrippsRoy KirschBenjamin WeissKayoko FukumoriAileen BerasateguiHeiko Vogelsubject
0106 biological sciences0301 basic medicine010603 evolutionary biology01 natural sciencesGeneral Biochemistry Genetics and Molecular BiologyCell wall03 medical and health sciencesSymbiosisEnterobacteriaceaeGenome SizeBotanyExtracellularAnimalsPectinaseSymbiosisOrganismbiologyHost (biology)food and beveragesbiology.organism_classificationPhenotypeColeoptera030104 developmental biologyBiochemistryPectinsBacteriaGenome Bacterialdescription
Pectin, an integral component of the plant cell wall, is a recalcitrant substrate against enzymatic challenges by most animals. In characterizing the source of a leaf beetle’s (Cassida rubiginosa) pectin-degrading phenotype, we demonstrate its dependency on an extracellular bacterium housed in specialized organs connected to the foregut. Despite possessing the smallest genome (0.27 Mb) of any organism not subsisting within a host cell, the symbiont nonetheless retained a functional pectinolytic metabolism targeting the polysaccharide’s two most abundant classes: homogalacturonan and rhamnogalacturonan I. Comparative transcriptomics revealed pectinase expression to be enriched in the symbiotic organs, consistent with enzymatic buildup in these structures following immunostaining with pectinase- targeting antibodies. Symbiont elimination results in a drastically reduced host survivorship and a diminished capacity to degrade pectin. Collectively, our findings highlight symbiosis as a strategy for an herbivore to metabolize one of nature’s most complex polysaccharides and a universal component of plant tissues.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-12-01 | Cell |