Determination of total free amino acids with o-phthalaldehyde and N-acetyl-l-cysteine

6533b83afe1ef96bd12a77a4

RESEARCH PRODUCT

Determination of total free amino acids with o-phthalaldehyde and N-acetyl-l-cysteine

M.j.medina Hernández R. M. Villanueva Camañas M.c.garcía Alvarez-coque

subject

O-Phthalaldehyde Stereochemistry Chemistry Composition (visual arts)Isoleucine Free amino Spectroscopy N-acetyl-L-cysteine Analytical Chemistry Cysteine

description

Abstract A spectrophotometric procedure is proposed for the determination of total free amino acids after reaction with o-phthalaldehyde and N-acetyl- l -cysteine, using isoleucine as the reference. The procedure was applied to the analysis of five samples of widely different composition. Recoveries were 98–105%.

year	journal	country	edition	language
1990-12-01	Microchemical Journal

https://doi.org/10.1016/0026-265x(90)90057-c

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