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RESEARCH PRODUCT
The establishment of two rat colonic carcinomas in tissue culture
Wolfgang Muller-klieserHelmut E. GabbertW. MellinRoland MollC. D. Gerharzsubject
endocrine systemPathologymedicine.medical_specialtyCellBiologyPhenotypeIn vitroTissue culturemedicine.anatomical_structureCell cultureIn vivomedicineUndifferentiated carcinomaCytoskeletondescription
Two rat colonie carcinomas (DMH-Co-1 and DMH-Co-2) derived from dimethyl-hydrazine-induced metastasizing adenocarcinomas were established as permanent cell lines. By means of electron microscopy, immunofluorescence microscopy and biochemical analysis of cytoskeletal components, it has been shown that both tumor cell lines retain in vitro the phenotypic characteristics of the primary tumors. The in vitro growth properties revealed only minor differences between the two cell lines. After retransplantation in vivo, DMH-Co-2 gave rise to moderately differentiated adenocarcinomas, whereas the tumors arising from DMH-Co-1 exhibited a continuum of differentiation encompassing adenocarcinomas, undifferentiated carcinomas and squamous cell carcinomas. These permanent cell lines offer the opportunity for isolating divergent subpopulations by in vitro cloning and facilitate standardized experiments on their biological behaviour in vivo.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1987-12-01 | Virchows Archiv B Cell Pathology Including Molecular Pathology |