6533b850fe1ef96bd12a82ff
RESEARCH PRODUCT
Identification of Tumor-Associated Autoantigens With SEREX
Dirk UsenerÖZlem TüreciSandra SchneiderUgur Sahinsubject
CloningbiologyAntigenLytic cyclelawbiology.proteinAutoantibodyRecombinant DNAGenomic libraryAntibodyMolecular biologySerologylaw.inventiondescription
Serological analysis of tumor antigens by recombinant cDNA expression cloning (SEREX) allows the systematic cloning of tumor antigens recognized by the spontaneous autoantibody repertoire of cancer patients. For SEREX, cDNA expression libraries are constructed from fresh tumor specimens, packaged into lambda-phage vectors, and expressed recombinantly in Escherichia coli. Recombinant proteins expressed during the lytic infection of bacteria are transferred onto nitrocellulose membranes to be probed with diluted autologous patient serum for identification of clones reactive with high-titered IgG antibodies. This chapter describes the SEREX technology in detail.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-12-07 |