6533b850fe1ef96bd12a840c

RESEARCH PRODUCT

An isocratic high-pressure liquid chromatographic purification method for radioactively labeled deoxyribonucleoside triphosphates.

Rudolf K. ZahnHans-joachim Breter

subject

Deoxyribonucleosidechemistry.chemical_compoundHydrolysisIsocratic elutionChromatographychemistryPotassium phosphateHigh pressureDeoxyribonucleotidesPurification methodsGeneral Biochemistry Genetics and Molecular BiologyChromatography High Pressure Liquid

description

Abstract A method is described for the rapid purification of radioactively labeled deoxyribonucleoside tri­phosphates from their spontaneously emerging hydrolysis products deoxyribonucleoside diphosphate, deoxyribonucleoside monophosphate, and deoxyribonucleoside. The separations which are finished within 3 min or less are carried out on a 0.1X5 cm column filled with LiChrosorb-NH2 , using isocratic elution with 0.025 м potassium phosphate, pH 6 .8 , in a high-pressure liquid chromatograph at room temperature and a flow rate of 30 ml · h-1(flow velocity 63.7 cm·min-1).

yearjournalcountryeditionlanguage
1976-10-01Zeitschrift fur Naturforschung. Section C, Biosciences
10.1515/znc-1976-9-1012https://pubmed.ncbi.nlm.nih.gov/136124