6533b850fe1ef96bd12a842f

RESEARCH PRODUCT

Complete decontamination and regeneration of DNA purification silica colum

Marcello TagliaviaAldo NicosiaFabrizio Gianguzza

subject

ChromatographyOctoxynolBiophysicsFungal geneticsSilica decontaminationGenomic DNACell BiologyHuman decontaminationSaccharomyces cerevisiaeDNA separation by silica adsorptionSilicon DioxideBiochemistryDNA extractionPolymerase Chain Reactionchemistry.chemical_compoundgenomic DNAchemistrySpin column-based nucleic acid purificationNucleic acidGenome FungalParticle SizeDNA FungalMolecular BiologyDNAChromatography High Pressure Liquid

description

Silica columns are among the most used DNA purification systems, allowing a good yield of high-quality nucleic acids without organic extractions. Silica column regeneration protocols reported up to now to remove DNA traces are time-consuming, and their effectiveness on genomic DNA has not been demonstrated. Here we report a very rapid regeneration procedure that ensures no DNA carryover, independent of its size, without impairing column efficiency. The method takes advantage of the improved DNA removal by low concentrations of Triton X-100.

yearjournalcountryeditionlanguage
2008-09-22
10.1016/j.ab.2008.10.021http://hdl.handle.net/10447/35824