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RESEARCH PRODUCT
Different deoxyribonucleases in human lymphocytes
Rudolf K. ZahnJorn BeckE. Jürgen ZöllnerWolfgang HelmManfred Reltzsubject
Malechemistry.chemical_classificationDeoxyribonucleasesHot TemperatureSubstrate (chemistry)DeoxyribonucleaseHydrogen-Ion ConcentrationIn Vitro TechniquesBiologyDivalentElectrophoresischemistry.chemical_compoundchemistryBiochemistryGeneticsHumansElectrophoresis Polyacrylamide GelLymphocytesDeoxyribonuclease IDeoxyribonucleasesIncubationDNAdescription
Abstract The distribution pattern of deoxyribonuclease activities in human lymphocytes has been examined by micro-disc-electrophoresis. Four groups of deoxyribonuclease activities, differing in their electrophoretic mobility, in the nature of their optimal substrate and in their optimal incubation conditions, are characterized. There are two alkaline DNase-activities. One corresponds to DNase I (EC 3.1.4.5), the other having pH optimum of about pH 9.0, prefers denatured DNA as substrate and is not dependent on divalent cations. The fractions with an acid pH optimum can be subdivided into two groups, which differ in their activity towards native DNA, towards denatured DNA, in their activity when succinate is present and in their pH optimum.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1974-08-01 | Nucleic Acids Research |