6533b851fe1ef96bd12a8c95

RESEARCH PRODUCT

Assessment and optimization of system parameters in size exclusion separation of proteins on diol-modified silica columns

P. RoumeliotisK.k. Unger

subject

ChromatographyResolution (mass spectrometry)fungiOrganic ChemistrySize-exclusion chromatographyDiolAnalytical chemistryfood and beveragesIonic bondingGeneral MedicineBiochemistryAnalytical Chemistrychemistry.chemical_compoundColumn chromatographychemistrySystem parameters

description

Abstract On diol-modified silica columns the retention of proteins is governed by a size exclusion effect, but superimposed on this are some secondary effects, i.e. , ionic and diol-ligand interactions which can be controlled and adjusted reproducibly by varying the eluent composition. The eluent composition also affects the column efficiency and peak shape. Both dependences can be employed to obtain a better resolution of proteins than can be expected from size exclusion alone.

yearjournalcountryeditionlanguage
1981-11-01Journal of Chromatography A
https://doi.org/10.1016/s0021-9673(00)82078-6