6533b851fe1ef96bd12a8ea0
RESEARCH PRODUCT
Streptococcus lactarius sp. nov., isolated from breast milk of healthy women.
Antonio Maldonado-barragánAntonio Maldonado-barragánRosario Mañes-lázaroJuan M. RodríguezVirginia Martínsubject
DNA BacterialPhylogenetic treebiologyMilk HumanStreptococcusLactobacillalesMolecular Sequence DataBacterialStreptococcusGeneral Medicine16S ribosomal RNAmedicine.disease_causebiology.organism_classificationStreptococcaceaeMicrobiologyMicrobiologyStreptococcus perorisStreptococcus mitisLactariusRNA Ribosomal 16SmedicineHumansFemaleEcology Evolution Behavior and SystematicsPhylogenydescription
Three strains of a hitherto-unknown, Gram-stain-positive coccus were recovered from the milk of three non-related healthy women. The isolates shared 99% 16S rRNA gene sequence similarity with sequences from uncultured members of the Lactobacillales and Streptococcus. The closest sequence corresponding to a defined species was that of Streptococcus peroris GTC 848T, with a similarity of 98%. A partial sequence (488 bp) of the tuf gene also showed 97% similarity with that of S. peroris CCUG 39814T. The combined 16S rRNA/tuf-based phylogeny revealed that all the isolates grouped in a statistically well-supported cluster separate from S. peroris. Enzyme activity profiles as well as fermentation patterns differentiated the novel bacteria from other members of the Streptococcus mitis group. Finally, phenotypic, genotypic and phylogenetic data supported the proposal of a novel species of the genus Streptococcus, for which the name Streptococcus lactarius sp. nov. is proposed. The type strain is MV1T (=CECT 7613T =DSM 23027T). © 2011 IUMS Printed in Great Britain.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-05-01 | International journal of systematic and evolutionary microbiology |