Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

6533b851fe1ef96bd12a943b

RESEARCH PRODUCT

Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

Arash Ghodousi S. Vatani D. Darban-sarokhalil M. Omrani A. A. Fooladi A. D. Khosaravi M. M. Feizabadi

subject

Settore MED/07 - Microbiologia E Microbiologia Clinica lcsh:QR1-502 Original Article Pulsed-filed gel electrophoresis Mycobacterium tuberculosis Mycobacterium tuberculosis TB-complex Pulsed-filed gel electrophoresis lcsh:Microbiology TB-complex

description

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.

year	journal	country	edition	language
2012-03-01

http://www.scopus.com/inward/record.url?eid=2-s2.0-84863827032&partnerID=MN8TOARS

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