Probing in cell protein structural changes with time-resolved X-ray scattering

6533b852fe1ef96bd12aad66

RESEARCH PRODUCT

Probing in cell protein structural changes with time-resolved X-ray scattering

Alessandro Spilotros Matteo Levantino Marco Cammarata Antonio Cupane Giorgio Schirò Michael Wulff

subject

Model system 010402 general chemistry 01 natural sciences law.invention 03 medical and health sciences law 030304 developmental biology 0303 health sciences Chemistry Scattering X-ray Time resolution in cell studie General Chemistry Nanosecond X-ray scattering Condensed Matter Physics Laser Conformational change Settore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)0104 chemical sciences Crystallography Chemical physics Allosteric transition Protein dynamic sense organs [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]

description

International audience; Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution.

year	journal	country	edition	language
2012-01-01

10.1039/c2sm25676b http://hdl.handle.net/10447/63231

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