6533b852fe1ef96bd12ab729

RESEARCH PRODUCT

Tracking the structural dynamics of proteins in solution using time-resolved wide-angle X-ray scattering

Michael WulffJungkweon ChoiAntonio CupaneFriedrich SchotteHyotcherl IheeFriederike EwaldMatteo LevantinoPhilip A. AnfinrudMarco Cammarata

subject

Materials scienceProtein ConformationCrystallography X-RayBiochemistrySensitivity and SpecificityArticlechemistry.chemical_compoundHemoglobinsProtein structureScattering RadiationSpectroscopyWide-angle X-ray scatteringMolecular Biologyprotein dynamics conformational changes hemoglobin myoglobin cytochrome cScatteringMyoglobinX-RaysResolution (electron density)Cytochromes cCell BiologyNanosecondMyoglobinchemistryChemical physicsProtein quaternary structuresense organsBiotechnology

description

We demonstrate tracking of protein structural changes with time-resolved wide-angle X-ray scattering (TR-WAXS) with nanosecond time resolution. We investigated the tertiary and quaternary conformational changes of human hemoglobin under nearly physiological conditions triggered by laser-induced ligand photolysis. We also report data on optically induced tertiary relaxations of myoglobin and refolding of cytochrome c to illustrate the wide applicability of the technique. By providing insights into the structural dynamics of proteins functioning in their natural environment, TR-WAXS complements and extends results obtained with time-resolved optical spectroscopy and X-ray crystallography.

yearjournalcountryeditionlanguage
2008-09-21
10.1038/nmeth.1255https://europepmc.org/articles/PMC3159148/