6533b852fe1ef96bd12ab863

RESEARCH PRODUCT

DNA Extraction From Orthoptera Museum Specimens

Bruno MassaMarcello TagliaviaMario La FarinaIda Albanese

subject

biologyChemistryOrthopteraBiochemistry (medical)Clinical BiochemistryGenetic markers; DNA extraction; DNA amplificationDna amplificationbiology.organism_classificationBiochemistryDNA extractionMolecular biologyMolecular taxonomyAnalytical Chemistrylaw.inventionchemistry.chemical_compoundDNA amplificationGenetic markerlawElectrochemistryGenetic markerDNA extractionSpectroscopyPolymerase chain reactionDNA

description

We describe a procedure for rapid purification of high quality DNA from either fresh or dry Orthoptera, suitable for the PCR amplification of DNA regions more than 800 bp long (even from oldest specimens), which allows genetic analyses on animals from collections without the complete specimen disruption.

https://doi.org/10.1080/00032719.2010.506939