Hepatīta B vīrusa nukleokapsīdu produkcija zīdītāju šūnās un to īpašību raksturojums

6533b852fe1ef96bd12abb17

RESEARCH PRODUCT

Hepatīta B vīrusa nukleokapsīdu produkcija zīdītāju šūnās un to īpašību raksturojums

Alma Eihentāle

subject

description

Lielākā daļa zināšanu par hepatīta B vīrusa kapsīdas (HBc) proteīnu ir balstītas uz pētījumiem par E.coli šūnās ekspresēto HBc. Zīdītāju šūnās ekspresēta HBc un kapsīdu pētījumi ļautu papildināt esošās zināšanas, kas varētu veicināt tālākus pētījumus un atklājumus. Šī darba ietvaros tika analizēta HBc proteīna ekspresija un lokalizācija CHO šūnās, no CHO šūnām ekspresētās kapsīdas tika attīrītas, noteikts kapsīdu blīvums, noteikta un salīdzināta fosforilācija dažādiem HBc rekombinantajiem analogiem un veikta natīvā elektroforēze kapsīdām. Tika raksturots T=3 un T=4 simetrijas kapsīdu saturs CHO-C6 šūnās ar elektronu mikroskopijas analīzi. Parādīts, ka CHO-C6 šūnās galvenokārt tiek ekspresētas kapsīdas ar blīvumu, kas atbilst tukšām kapsīdām. Kapsīdu nukleīnskābju saturs tika pārbaudīts arī ar natīvas elektroforezes metodi. Atslēgvārdi: HBc, eikariotiskā ekspresija, CHO šūnas

year	journal	country	edition	language
2014-01-01

https://dspace.lu.lv/dspace/handle/7/20976

PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
  - PE2_1Theory of fundamental interactions
  - PE2_2 Phenomenology of fundamental interactions
  - PE2_3Experimental particle physics with accelerators
  - PE2_4Experimental particle physics without accelerators
  - PE2_6Nuclear, hadron and heavy ion physics
  - PE2_8Gas and plasma physics
  - PE2_5Classical and quantum physics of gravitational interactions
  - PE2_7Nuclear and particle astrophysics
  - PE2_9 Electromagnetism
  - PE2_10Atomic, molecular physics
  - PE2_11Ultra-cold atoms and molecules
  - PE2_18Equilibrium and non-equilibrium statistical mechanics: steady states and dynamics
  - PE2_14Lasers, ultra-short lasers and laser physics
  - PE2_17Metrology and measurement
  - PE2_16Non-linear physics
  - PE2_12Optics, non-linear optics and nano-optics
  - PE2_13Quantum optics and quantum information
  - PE2_15Thermodynamics
- PE1Mathematics
  - PE1_1Logic and foundations
  - PE1_6Geometry and global analysis
  - PE1_8Analysis
  - PE1_7Topology
  - PE1_9Operator algebras and functional analysis
  - PE1_10ODE and dynamical systems
  - PE1_12Mathematical physics
  - PE1_11Theoretical aspects of partial differential equations
  - PE1_13Probability
  - PE1_14Mathematical statistics
  - PE1_15Generic statistical methodology and modelling
  - PE1_4 Algebraic and complex geometry
  - PE1_19Scientific computing and data processing
  - PE1_2Algebra
  - PE1_20Control theory, optimisation and operational research
  - PE1_21Application of mathematics in sciences
  - PE1_22Application of mathematics in industry and society
  - PE1_5Lie groups, Lie algebras
  - PE1_18Numerical analysis
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  - PE1_17Mathematical aspects of computer science
  - PE1_3Number theory
- PE3Condensed Matter Physics
  - PE3_1Structure of solids, material growth and characterisation
  - PE3_2Mechanical and acoustical properties of condensed matter, lattice dynamics
  - PE3_3Transport properties of condensed matter
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  - PE11_12Semi-conducting and magnetic materials engineering
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  - PE11_14Computational methods for materials engineering
LSLife Sciences
- LS1Molecules of Life: Biological Mechanisms, Structures and Functions
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  - LS1_9Molecular mechanisms of signalling processes
  - LS1_7Molecular biophysics, biomechanics, bioenergetics
- LS2Integrative Biology: from Genes and Genomes to Systems
  - LS2_1Genetics
  - LS2_3Epigenetics
  - LS2_2Gene editing
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  - LS2_7Transcriptomics
  - LS2_8Proteomics
  - LS2_9Metabolomics
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- LS4Physiology in Health, Disease and Ageing
  - LS4_1Organ and tissue physiology and pathophysiology
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- LS3Cell Biology, Development, Stem Cells and Regeneration
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- LS9Biotechnology and Biosystems Engineering
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SHSocial Sciences and Humanities
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