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RESEARCH PRODUCT

Recherche de critères phénotypiques et moléculaires discriminants des différentes espèces et sous-espèces du genre Pisum

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The Pisum genus presents a large genetic diversity, that makers difficult its taxonomic classification. The Center for Grain Legumes Genetic Resources holds 1800 pea accessions. Some of them are lacking passport data. Previous genotyping studies have identified SNP markers specific to wild Pisum pools. The main aim of my work was to conceive simple criteria in order to classify the unknown genotypes. Towards this goal, plants have been observed at the phenotypic level and their DNA were extracted for their characterisation using discriminating SNP markers. Furthermore, candidate genes of domestication were sequenced. Our results clearly showed distinctive traits between the P. sativum and P. fulvum accessions. Among these criteria, P. sativum had larger flower peduncles and stipules than P. fulvum. The latter had dented leaflets and orange flowers. Wild sub-species including P.sativum elatius, humile, and abyssinicum as well as P. fulvum had curved and dehiscent pods. Some tested SNP were specific to the different wild pools. In particular, the gene PsCam023451 showed mutations in the coding sequence, and the gene Pscam044473 had two mutation sites fixed in P. fulvum. Genome size was significantly different between P. fulvum and P. sativum.