6533b853fe1ef96bd12ac3fa

RESEARCH PRODUCT

Phenotypic and genotypic evaluation of slime production by conventional and molecular microbiological techniques.

Liberto McMatera GQuirino ALamberti AgCapicotto RPuccio RBarrecaGsFoca ECascio A.Foca A

subject

Coagulase-negative staphylococci; Ica genes; Real-time PCR; Slime; Bacterial Capsules; Bacterial Proteins; Bacteriological Techniques; Genotype; Humans; Phenotype; Polymerase Chain Reaction; Staining and Labeling; Staphylococcal Infections; Staphylococcus; MicrobiologyGenotypeICADStaphylococcusBiologySlimeMicrobiologyPolymerase Chain ReactionMicrobiologyAgar plateBacterial ProteinsGenotypeGene expressionHumansGeneBacterial CapsulesBacteriological TechniquesIca genesStaining and LabelingCoagulase-negative staphylococciStaphylococcal InfectionsPhenotypeMolecular biologyReal-time polymerase chain reactionPhenotypeSlime Real-time PCR Coagulase-negative staphylococci Ica genesCoagulaseReal-time PCR

description

Twenty-nine staphylococcal isolates from different clinical samples were tested for slime production: phenotypic characterization was carried out using Christensen test (tube test) and Congo red agar plate test (CRA plate test), while the presence and expression of icaA and icaD genes were evaluated by real-time PCR. In 79.3% of studied strains there was a concordance between slime production and presence of icaA and icaD genes, and between lack of slime production and absence of both or only one of the tested genes. In four of five strains where positive phenotype was not associated with the presence of ica genes, gene co-expression (evaluated by mRNA determination) was lacking, while in only a case out of five, there was the presence of transcripts without phenotype. Our study, for the first time, introduces real-time PCR for the detection of both icaA and icaD genes and their mRNA and, furthermore, addresses the relationship between slime phenotype absence and mRNA expression, in clinical isolates of coagulase-negative staphylococci.

yearjournalcountryeditionlanguage
2009-09-01Microbiological research
10.1016/j.micres.2007.04.004https://pubmed.ncbi.nlm.nih.gov/17928210