6533b853fe1ef96bd12acb7c
RESEARCH PRODUCT
Determination of 14-3-3 protein levels in cerebrospinal fluid from Creutzfeldt-Jakob patients by a highly sensitive capture assay.
Katell Peoc'hJean-louis LaplancheWerner E. G. M�llerHeinz C. SchröderSanja Ramljaksubject
Tyrosine 3-MonooxygenaseImmunoblottingPeptideEnzyme-Linked Immunosorbent AssayPlasma protein bindingBiologySensitivity and SpecificityCreutzfeldt-Jakob SyndromeCerebrospinal fluidmental disordersHumansIn patientPhosphorylation14-3-3 proteinchemistry.chemical_classificationGeneral NeuroscienceCreutzfeldt-Jakob SyndromeVirologyMolecular biologynervous system diseasesHighly sensitivechemistry14-3-3 ProteinsChemistry ClinicalPhosphorylationProtein Bindingdescription
The level of 14-3-3gamma protein was determined in the cerebrospinal fluid (CSF) from patients with Creutzfeldt-Jakob disease (CJD) and non-CJD patients applying a new and fast microplate assay (14-3-3 protein capture assay), based on the binding to a peptide comprising a phosphorylated recognition motif of 14-3-3 protein. The levels of the gamma-isoform of 14-3-3 protein in CSF samples from CJD patients (n=41) were significantly higher than those observed in patients with non-CJD dementias (n=36) suggesting that this capture assay is a reliable method in the diagnosis of CJD. Since this assay allows the direct measurement of 14-3-3 protein in the CSF without prior concentration it is an easy and simple alternative to the conventionally applied immunoblotting procedures.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-04-01 | Neuroscience letters |