6533b853fe1ef96bd12acb8a
RESEARCH PRODUCT
Evaluation of immunologic effect of Enniatin A and quantitative determination in feces, urine and serum on treated Wistar rats.
Lara ManyesGuillermina FontCristina JuanAna Juan-garcíasubject
Malemedicine.medical_specialtyLymphocyteCD4-CD8 RatioUrineBiologyToxicologychemistry.chemical_compoundEatingFecesAntigenIn vivoInternal medicineDepsipeptidesmedicineCytotoxic T cellAnimalsLymphocytesRats WistarMycotoxinFecesBody WeightDietRatsEndocrinologymedicine.anatomical_structurechemistryCD8description
Abstract Study of dietary supplementation with ENN A mycotoxin during 28 days of exposure time on Wistar rats to determinate its levels in serum, urine and feces and, to evaluate the immunologic effect in peripheral blood lymphocytes (PBL) is presented. The first method for ENN A extraction, determination and detection by LC–MS/MS in serum, urine and feces samples is reported. ENN A food dose administrated was detected in serum samples and influenced lymphocyte phenotyping. Levels in serum were founded from the second week of the experiment; reaching values of 4.76 μg/ml on the fourth week, which corresponds to 3.24 μg/ml in blood. PBL as T helper (CD4 + ) were presented in greater percentages compared to control ( p ≤ 0.001), while T cytotoxic (CD8 + ) decreased significantly compared to control ( p ≤ 0.001). ENN A treatment significantly increased CD4 + /CD3 + and CD4 + /CD8 + ratios but significantly decreased CD8 + /CD3 + ratio. CD4 + /CD8 + ratio was 2.94:1, indicating that PBL surface antigen expression and immune status in Wistar rats treated were impaired by the ENN A mycotoxin.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-03-19 | Toxicon : official journal of the International Society on Toxinology |