6533b853fe1ef96bd12acca5

RESEARCH PRODUCT

Fast analysis of polyphenols and alkaloids in cocoa-based products by ultra-high performance liquid chromatography and Orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap-MS/MS).

Giulia GrazianiAlberto RitieniAntonello SantiniAnna GaspariYelko Rodríguez-carrasco

subject

PolyphenolHigh-resolution mass spectrometryRelative standard deviationOrganolepticOrbitrapMass spectrometry01 natural scienceslaw.inventionQ-Exactive Orbitrap0404 agricultural biotechnologyCocoaAlkaloidslawTandem Mass SpectrometryAlkaloidStructural isomerFood analysis.Chromatography High Pressure LiquidCacaoChromatographyChemistry010401 analytical chemistryOrbitrap msUltra-high performance liquid chromatographyPolyphenols04 agricultural and veterinary sciences040401 food science0104 chemical sciencesPolyphenolUltra high performanceFood Science

description

The cocoa varieties Criollo, Forastero and Trinitario, which have different organoleptic properties, quite often are mixed together in cocoa-based products. The objective of this work was to develop a rapid method for the simultaneous determination of polyphenols (n = 35) and alkaloids (n = 2) in cocoa-based products by using the high throughput advantages provided by the Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (FIRMS). The proposed procedure was optimized and validated in terms of selectivity and specificity (mass accuracy 0.990), accuracy (recovery range from 89.1 to 112.4%) and precision (relative standard deviation < 10%). The method was applied to 80 cocoa-based samples. Alkaloids represented on average the 57.7% of assayed compounds. Flavanols and procyanidins were the most abundant polyphenols being quantified in a range between 261 and 668 mg/kg and from 127 to 405 mg/g, respectively. Mass equivalents but structural isomers bioflavonoids were characterized by using the HRMS-Orbitrap methodology here developed.

yearjournalcountryeditionlanguage
2018-09-01Food research international (Ottawa, Ont.)
10.1016/j.foodres.2018.05.032https://pubmed.ncbi.nlm.nih.gov/30007681