6533b853fe1ef96bd12acd10
RESEARCH PRODUCT
Timm-staining intensity is correlated with the density of Timm-positive presynaptic structures in the cerebral cortex of lizards
A. MolownyCarlos Lopez-garciaFrancisco José Martínez-guijarrosubject
HistologyPodarcis hispanicaSynaptic vesicleTimm stainingmedicineAnimalsMolecular BiologyCerebral CortexStaining and LabelingbiologyVesicleLizardsCell BiologyGeneral MedicineAnatomybiology.organism_classificationIntensity (physics)Reaction productMicroscopy ElectronMedical Laboratory Technologymedicine.anatomical_structureCerebral cortexUltrastructureRegression AnalysisSynaptic VesiclesAnatomyGeneral Agricultural and Biological SciencesDensitometrydescription
In cortical areas of the lizard, Podarcis hispanica, Timm staining reveals a distinct pattern of lamination. At the electron-microscope level, virtually all of the reaction product is located in the synaptic vesicles of Timm-positive boutons. Using linear-regression analysis, the area density of Timm-positive bouton profiles as well as the numerical and volume density of stained vesicles were found to be closely correlated with the light-microscopic densitometric values obtained for each Timm-positive cortical zone. We discuss the possibility of estimating stereological electron-microscopic data parameters from densitometric measurements at the light-microscope level.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1987-01-01 | Histochemistry |