6533b853fe1ef96bd12ace38
RESEARCH PRODUCT
Odor-induced electrical and calcium signals from olfactory sensory neurons in situ
Xavier GrosmaitreMinghong Masubject
0301 basic medicineSensory systemGCaMP6gene targeting03 medical and health sciences0302 clinical medicineCalcium imagingolfactory sensory neuronsmedicinePatch clampCalcium signalingChemistryrespiratory systempatch-clampelectrophysiologytransductionElectrophysiologycalcium imaging030104 developmental biologymedicine.anatomical_structureOdor[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]sense organsNeuroscienceTransduction (physiology)Olfactory epithelium030217 neurology & neurosurgerydescription
Electrophysiological recording and optical imaging enable the characterization of membrane and odorant response properties of olfactory sensory neurons (OSNs) in the nasal neuroepithelium. Here we describe a method to record the responses of mammalian OSNs to odorant stimulations in an ex vivo preparation of intact olfactory epithelium. The responses of individual OSNs with defined odorant receptor types can be monitored via patch-clamp recording or calcium imaging.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-01-01 |