6533b853fe1ef96bd12ad4e7
RESEARCH PRODUCT
rDNA (18S-28S and 5S) co-localization and linkage between ribosomal genes and (TTAGGG)n telomeric sequence in the earthworm Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae) revealed by single- and double-colour FISH
A.m. PirroneMauro MandrioliRoberto VitturiMariastella Colombasubject
Genetic LinkageDNA Ribosomalchemistry.chemical_compoundbiology.animalGeneticsAnimalsLumbricidaeOligochaetaRepeated sequenceMolecular BiologyGeneIn Situ Hybridization FluorescenceGenetics (clinical)fishbiologyEcologyEarthwormTelomereRibosomal RNAbiology.organism_classificationMolecular biologyTelomerechemistryOligochaetaDNABiotechnologydescription
Spermatogonial and metaphase I chromosomes of the lumbricid earthworm Octodrilus complanatus (Annelida: Oligochaeta) were examined using fluorescent in situ hybridization (FISH) with three repetitive DNA probes-5S rDNA, 18S-26S rDNA, and (TTAGGG)(n). Single-color FISH consistently mapped one chromosome pair per spread using either 5S rDNA or 18S-26S rDNA as probes. Simultaneous (18S-26S)-5S and (18S-26S)-(TTAGGG)(n) FISH demonstrated that repeated units of the two ribosomal families were overlapped and closely associated with telomeric sequences.
year | journal | country | edition | language |
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2002-01-01 |