6533b853fe1ef96bd12ad4ec
RESEARCH PRODUCT
Molecularly imprinted polymer-based device for field collection of oral fluid samples for cocaine identification.
José Manuel Herrero-martínezA. Sorribes-sorianoSergio ArmentaFrancesc A. Esteve-turrillassubject
ChromatographyElutionIon-mobility spectrometryChemistryPolymers010401 analytical chemistryOrganic ChemistryMolecularly imprinted polymerGeneral Medicine010402 general chemistry01 natural sciencesBiochemistry0104 chemical sciencesAnalytical ChemistryMolecular ImprintingSubstance Abuse DetectionHealth personnelIdentification (information)CocaineIon Mobility SpectrometryOral fluidHumansSample collectionSalivaLateral flow immunoassaydescription
In this paper, a low-cost, rapid, easy, and potentially portable tool for the identification of cocaine and its semi-quantitative determination in oral fluid has been proposed. A field collection device has been designed, based on a cotton pad with an indicator and a molecularly imprinted polymer (MIP) sorbent, to selective retain cocaine from oral fluid components. After sample collection, cocaine is transferred by using phosphate buffer to the MIP and then eluted with 2-propanol. The obtained extract is analysed by ion mobility spectrometry (IMS), providing a cut-off value of 20 µg L-1 that identifies 100 % true-positive and 95 % true-negative samples. The MIP-IMS procedure has been validated by the analysis of oral fluid samples, collected from cocaine users at recreation environments, by comparing the results with lateral flow immunoassay and chromatographic reference methods. Thus, the proposed methodology allows a simple and fast cocaine identification that can be carried out in field by non-specialized personnel, such as health personnel, law enforcement bodies, and customs staff.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-09-09 | Journal of chromatography. A |