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RESEARCH PRODUCT

Biphasic 24-Hour Variations in Cyclic GMP Accumulation in the Rat Pineal Gland Are Due to Corresponding Changes in the Activity of Cytosolic and Particulate Guanylate Cyclase

Lutz VollrathRainer SpessertBraj B.p. GuptaKatja Heil

subject

Malemedicine.medical_specialtyTime FactorsBiologyArginineNitric OxidePineal GlandBiochemistryNitric oxideRats Sprague-DawleyCellular and Molecular NeurosciencePineal glandchemistry.chemical_compoundCytosolInternal medicinemedicineAnimalsCircadian rhythmCyclic GMPchemistry.chemical_classificationomega-N-MethylargininePhosphodiesteraseMetabolismCircadian RhythmRatsEnzyme ActivationCytosolEnzymeEndocrinologymedicine.anatomical_structurechemistryBiochemistryGuanylate CyclaseEndocrine gland

description

Various parameters of the rat pineal gland display a 24-h rhythm. However, nothing is known about possible 24-h variations in cyclic GMP (cGMP) metabolism. In the present study, 24-h variations in pineal gland cGMP accumulation were investigated by determining the increase in cGMP level with and without inhibitors of phosphodiesterase at different time points over a light/dark cycle (12/12 h). Furthermore, the activity of guanylate cyclase (GC) was determined under substrate-saturated conditions regarding the cytosolic and particulate forms of the enzyme. It has been found that cGMP accumulation and GC activity display biphasic 24-h variations with two peaks--one approximately 7 h after lights "on" and the other approximately 7 h after lights "off." The activity of cytosolic GC remains unchanged in the presence of the nitric oxide (NO) synthesis inhibitor N-monomethyl-L-arginine, indicating that 24-h variations in the activity do not reflect changes in the synthesis of the GC stimulator NO.

yearjournalcountryeditionlanguage
2006-10-05Journal of Neurochemistry
https://doi.org/10.1111/j.1471-4159.1992.tb10098.x