Studies on Holothuriapolii (echinodermata) coelomocyte lysate II. Isolation of coelomocyte hemolysins

6533b854fe1ef96bd12ae1c8

RESEARCH PRODUCT

Studies on Holothuriapolii (echinodermata) coelomocyte lysate II. Isolation of coelomocyte hemolysins

Calogero Canicattì Donatella Ciulla

subject

Gel electrophoresis Immunodiffusion biology Sea Cucumbers Immunology Hemolysin biology.organism_classification Hemolysin Proteins Microbiology Molecular Weight Hemolysin Proteins Cytolysis Red blood cell medicine.anatomical_structure Lytic cycle medicine Animals Electrophoresis Polyacrylamide Gel Holothuria Coelomocyte Echinodermata Developmental Biology

description

The lytic activity of the Holothuria polii coelomocyte lysate resides in two electrophoretically distinct hemolysins identified as He1 and He2. He1 represents the calcium dependent, heat-labile component whereas He2 is calcium independent and heat-stable. The two hemolysins share serological identity. Both hemolysins appear as single protein molecules of 80KDa molecular weight by SDS-PAGE and transblotting analysis under non-reducing conditions. However under reducing conditions, they are doublets of 76 and 80KDa molecular weight. The hypothesis that the two hemolysins could be isoforms is discussed.

year	journal	country	edition	language
1988-12-01	Developmental & Comparative Immunology

https://doi.org/10.1016/0145-305x(88)90024-9

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