6533b854fe1ef96bd12aea9e

RESEARCH PRODUCT

Zygocotyle lunata: Proteomic analysis of the adult stage

Bernard FriedRafael ToledoM. Luz ValeroJavier SotilloManuel M. Sánchez Del PinoJ. Guillermo EstebanAntonio Marcilla

subject

MaleProteomeProtein familySomatic cellImmunologyTrematode InfectionsBiologyBioinformaticsTandem mass spectrometryHost-Parasite InteractionsMiceTandem Mass SpectrometryAnimalsParasite hostingDatabase search engineParamphistomatidaeDatabases ProteinMice Inbred BALB COxygen transportHelminth ProteinsGeneral Medicinebiology.organism_classificationInfectious DiseasesBiochemistryProteomeParasitologyTrematodaChromatography Liquid

description

The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated using a proteomic approach to separate and identify tryptic peptides from the somatic extract of Z. lunata adult worms. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 36 proteins were accurately identified from the worms. The largest protein family consisted of metabolic enzymes. Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. lunata. This is the first study that attempts to identify the proteome of Z. lunata. However, more work is needed to improve our knowledge of trematodiasis in general and more specifically to have a better understanding about host-parasite relationships in infections with paramphistomes.

yearjournalcountryeditionlanguage
2011-06-01Experimental Parasitology
https://doi.org/10.1016/j.exppara.2011.02.014