Citohroma c peroksidāzes loma Zymomonas Mobilis termiskā un oksidatīvā stresa noturībā

6533b854fe1ef96bd12aedd6

RESEARCH PRODUCT

Citohroma c peroksidāzes loma Zymomonas Mobilis termiskā un oksidatīvā stresa noturībā

subject

Zymomonas mobilis cytochrome c peroxidase oxidative stress Bioloģija thermal stress

description

Lai pētītu Zymomonas mobilis citohroma c peroksidāzes ietekmi uz celmu termiskā un oksidatīvā stresa noturību, izveidots plazmīdas konstrukts ar ccp gēnu. Ar to transformēts Zymomonas mobilis celms Zm6. Kultivējot jauniegūto celmu, izejas celmu un iepriekš izveidotus mutantus celmus ar inaktivētu citohroma c peroksidāzi un celmu ar inaktivētu katalāzi, salīdzināta celmu izturība oksidatīvā un termālā stresa apstākļos. Audzēšanas eksperimentos parādīts, ka pārbaudītajos mikroaerofīlajos apstākļos citohroma c peroksidāzes aizsargājošā funkcija nav tik izteikta kā aerobos apstākļos. Mikroaerofīlos apstākļos aizsardzību pret reaktīvajām skābekļa formām primāri nodrošina katalāze

year	journal	country	edition	language
2020-01-01

https://dspace.lu.lv/dspace/handle/7/50005

PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
  - PE2_1Theory of fundamental interactions
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- PE1Mathematics
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LSLife Sciences
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SHSocial Sciences and Humanities
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