6533b854fe1ef96bd12af1e7

RESEARCH PRODUCT

Outbreak of CTX-M-15-producing Klebsiella pneumoniae of sequence type 199 in a Latvian teaching hospital

Aina IversenEdvīns MiklaševičsMāra SauleArta BalodeChristian G. GiskeUga Dumpis

subject

Microbiology (medical)biologyKlebsiella pneumoniaemedicine.medical_treatmentOutbreakGeneral Medicinebiochemical phenomena metabolism and nutritionbacterial infections and mycosesbiology.organism_classificationVirologyEnterobacteriaceaePathology and Forensic MedicineMicrobiologychemistry.chemical_compoundchemistryPulsed-field gel electrophoresisBeta-lactamasemedicineImmunology and AllergyMultilocus sequence typingTypingErtapenem

description

Dumpis U, Iversen A, Balode A, Saule M, Miklasevics E, Giske CG. Outbreak of CTX-M-15-producing Klebsiella pneumoniae of sequence type 199 in a Latvian teaching hospital. APMIS 2010; 118: 713–6. Previous studies on the epidemiology of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in Latvia are lacking. ESBL-producing Klebsiella pneumoniae (n = 32) were subjected to pulsed-field gel electrophoresis (PFGE) and selected isolates to multi-locus sequence typing (MLST). Species identification and susceptibility testing were performed using VITEK2, and sequencing of blaCTX-M was performed in selected isolates. PFGE revealed one major clone (n = 23), with most of the isolates derived from the ICU. The clone harboured blaCTX-M-15, was sequence type 199 and comprised two ertapenem non-susceptible isolates. This is the first report of an ESBL outbreak in Latvia, and calls for increased epidemiological typing of ESBL-producing Enterobacteriaceae, as well as improved infection control routines.

yearjournalcountryeditionlanguage
2010-08-18APMIS
https://doi.org/10.1111/j.1600-0463.2010.02640.x