6533b854fe1ef96bd12af490
RESEARCH PRODUCT
Evaluation of a mass spectrometry and Vitek 2 combined protocol for rapid identification and susceptibility testing of Enterobacterales directly from positive blood cultures.
Begoña FusterMaria Del Remedio GunaManuel BeldaNuria TormoConcepción GimenoMiriam Torrecillassubject
0301 basic medicineMicrobiology (medical)medicine.medical_specialtySusceptibility testing030106 microbiologyAntimicrobial susceptibilityBacteremiaMicrobial Sensitivity Tests03 medical and health sciences0302 clinical medicineSpecies levelEnterobacteriaceaeInternal medicineEnterobacteralesmedicineHumans030212 general & internal medicineProtocol (science)business.industryAnti-Bacterial AgentsRapid identificationCiprofloxacinAmikacinBlood CultureSpectrometry Mass Matrix-Assisted Laser Desorption-Ionizationbusinessmedicine.drugdescription
Abstract Objective The aim was to evaluate a rapid method which would combine identification and susceptibility testing directly from positive blood cultures for Gram-negative bacilli of the Enterobacterales. Material and methods Gram-negative rods from blood cultures were directly identified by MALDI-TOF. Samples with Enterobacterales were selected for direct antimicrobial susceptibility testing by Vitek 2. The results were compared to those obtained with our laboratory's standard method. Results MALDI-TOF directly from blood cultures identified correctly 83% of the samples. Enterobacterales (n = 68) were identified at gender and species level in 85% of blood cultures with a score >1.7. In general, MICs were obtained after 7 h. MICs of amoxicillin-clavulanate, amikacin and ciprofloxacin showed in almost 50% of the cases after 5 h. Conclusions A simple procedure with low cost and reduced working time makes it possible to integrate both identification and susceptibility testing directly from blood cultures. Thus, this protocol could offer advantages when it comes to selection and cost of treatment and patients’ clinical outcomes.
year | journal | country | edition | language |
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2020-10-01 | Enfermedades infecciosas y microbiologia clinica (English ed.) |