6533b855fe1ef96bd12aff89
RESEARCH PRODUCT
Polyphasic identification of yeasts isolated from bark of cork oak during the manufacturing process of cork stoppers.
Federico UruburuMercedes Villa-carvajalCarmela BellochJuan José R. CoqueMaría Luisa ÁLvarez-rodríguezsubject
MycologyCorkengineering.materialRhodotorulaApplied Microbiology and BiotechnologyMicrobiologyDNA RibosomalPolymerase Chain ReactionRhodotorula mucilaginosaQuercusYeastsDebaryomyces hanseniiBotanyTrichosporon mucoidesDNA FungalMycological Typing TechniquesRibosomal DNAbiologyBase SequenceFungal geneticsGeneral Medicinebiology.organism_classificationPhenotypevisual_artvisual_art.visual_art_mediumengineeringPlant BarkBarkPolymorphism Restriction Fragment Lengthdescription
A two-step protocol was used for the identification of 52 yeasts isolated from bark of cork oak at initial stages of the manufacturing process of cork stoppers. The first step in the identification was the separation of the isolates into groups by their physiological properties and RFLPs of the ITS-5.8S rRNA gene. The second step was the sequencing of the D1/D2 domains of the 26S rRNA gene of selected isolates representing the different groups. The results revealed a predominance of basidiomycetous yeasts (11 species), while only two species represented the ascomycetous yeasts. Among the basidiomycetous yeasts, members representing the species Rhodosporidium kratochvilovae and Rhodotorula nothofagi, that have been previously isolated from plant material, were the most abundant. Yeasts pertaining to the species Debaryomyces hansenii var. fabryii, Rhodotorula mucilaginosa and Trichosporon mucoides were isolated in small numbers.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-06-18 | FEMS yeast research |