6533b855fe1ef96bd12b086b

RESEARCH PRODUCT

Transcription in bacteriophage f1-infected Escherichia coli: RNA synthesized on DNA of deletion mutant PII shows the existence of a two-site terminator.

Maria VitaleMario La FarinaVincenzo Enea

subject

Genes ViralTranscription GeneticBiologymedicine.disease_causeColiphagesBacteriophageNucleic acid thermodynamicschemistry.chemical_compoundTranscription (biology)GeneticsmedicineNucleotideMolecular BiologyEscherichia colichemistry.chemical_classificationBase SequenceRNAChromosome MappingNucleic Acid Hybridizationbiology.organism_classificationMolecular biologyTerminator (genetics)chemistryDNA ViralMutationNucleic Acid ConformationRNA ViralDNA

description

Two different transcripts are synthesized on the DNA of deletion mutant PII of bacteriophage f1 in E. coli cells infected with this miniphage. Both RNA species appear to be primary transcripts and differ by about 100 nucleotides at their 3'OH end. Mapping of these molecules on the miniphage genome suggests that a two-site terminator is active at the end of the I region of transcription of bacteriophage f1.

10.1007/bf00341441https://pubmed.ncbi.nlm.nih.gov/6590942