6533b855fe1ef96bd12b0872
RESEARCH PRODUCT
Enhanced hybridization labeling signals in Southern blotted DNAs fractionated with voltage gradient gel electrophoresis.
Rainer BarbieriVincenzo IzzoGiovanni Durosubject
Gel electrophoresisElectrophoresis Agar GelChromatographyGel electrophoresis of nucleic acidsClinical BiochemistryVoltage gradientMembrane ProteinsNucleic Acid HybridizationDNAChemical FractionationBiochemistryAnalytical Chemistrychemistry.chemical_compoundBlotting SouthernchemistryMolecular-weight size markerSea UrchinsNucleic acidPulsed-field gel electrophoresisElectrochemistryAnimalsDNASouthern blotdescription
An enhancement of hybridization labeling signals is demonstrated in Southern blotted DNAs, fractionated by voltage gradient gel electrophoresis. This enhancement is due to a reduced thickness of each single nucleic acid band in the gel as a consequence of the gradient effect, corresponding to an increased concentration of DNA per unit area.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-05-01 | Electrophoresis |