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RESEARCH PRODUCT
Semiquantitative bioluminescent assay of glutathione
Wolfgang Mueller-klieserFrancisco J. Romerosubject
chemistry.chemical_classificationBioluminescent assayChemistryGlutathione reductaseBiophysicsGlutathioneRedoxchemistry.chemical_compoundEnzymeBiochemistryChemistry (miscellaneous)FMN reductaseBioluminescenceLuciferasedescription
A novel technique has been developed for semiquantitative detection of glutathione (GSH) in small volumes of liquid samples. GSH is detected via enzymatic linkage to the NADP/NADPH + H+ redox system through glutathione reductase. Accumulated NADPH is measured via the bioluminescent FMN oxidoreductase bacterial luciferase reaction. A linear correlation is obtained between bioluminescence intensity of the luciferase reaction and the GSH content of the liquid sample. Possible applications of this procedure are discussed. © 1998 John Wiley & Sons, Ltd.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-09-01 | Journal of Bioluminescence and Chemiluminescence |