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RESEARCH PRODUCT
Separation of Proteic Primary Amino Acids under Several Reversed‐Phase Liquid Chromatographic Conditions
Jose Ramon Torres-lapasioV. Concha-herreraG. Vivó-truyolsM.c. García-alvarez-coquesubject
chemistry.chemical_classificationChromatographyResolution (mass spectrometry)ChemistryClinical BiochemistryPharmaceutical ScienceReversed-phase chromatographyBiochemistryHigh-performance liquid chromatographyMicelleAnalytical ChemistryAmino acidchemistry.chemical_compoundChromatography detectorTrifluoroacetic acidDerivatizationdescription
Abstract The reversed‐phase liquid chromatographic (RPLC) analysis of proteic primary amino acids with acetonitrile‐water, using pre‐column derivatisation with o‐phthalaldehyde (OPA) and N‐acetylcysteine (NAC), was compared with RPLC modes using trifluoroacetic acid or pentadecafluorooctanoic acid and evaporative light‐scattering detection, or sodium dodecyl sulphate micelles with pre‐ and post‐column derivatisation. The importance of column lifetime, risk of potential damages in the instrumentation, retention and resolution, was considered. Among the assayed approaches, the best is still aqueous‐organic RPLC with pre‐column derivatisation. It not only yields the most reliable results, but allows acceptable resolution and longer column lifetime, and its implementation is simpler.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-10-01 | Journal of Liquid Chromatography & Related Technologies |