6533b856fe1ef96bd12b1c45
RESEARCH PRODUCT
Fibronectin-binding nanoparticles for intracellular targeting addressed by B. burgdorferi BBK32 protein fragments.
Viesturs BaumanisViesturs BaumanisIrina SominskayaIvars PetrovskisVelta OseJanis BogansIrena TimofejevaInara AkopjanaRuta BruverePauls PumpensRenāte RankaRenate RankaKalvis Brangulissubject
:MEDICINE [Research Subject Categories]virusesBiomedical EngineeringPharmaceutical ScienceMedicine (miscellaneous)BioengineeringPeptideBiologyVirusPlasmidBacterial ProteinsCaveolaeGeneral Materials ScienceDNA Primerschemistry.chemical_classificationBase SequenceVirologyCell biologyFibronectins:NATURAL SCIENCES::Biology [Research Subject Categories]FibronectinchemistryCapsidFibronectin bindingBorrelia burgdorferibiology.proteinMolecular MedicineNanoparticlesIntracellularPlasmidsdescription
Virus-like particles (VLPs) are created by the self-assembly of multiple copies of envelope and/or capsid proteins from many viruses, mimicking the conformation of a native virus. Such noninfectious nanostructures are mainly used as antigen-presenting platforms, especially in vaccine research; however, some of them recently were used as scaffolds in biotechnology to produce targeted nanoparticles for intracellular delivery. This study demonstrates the creation of fusion VLPs using hepatitis B core protein-based system maintaining a fibronectin-binding property from B. burgdorferi BBK32 protein, including the evidence of particles’ transmission to BHK-21 target cells via caveolae/rafts endocythosis. These results make this construct to be an attractive model in development of HBc-based nanoparticles for cellular targeting applications and highlights the fragment of B. burgdorferi BBK32 as a novel cellular uptake-promoting peptide.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-12-22 | Nanomedicine : nanotechnology, biology, and medicine |