6533b856fe1ef96bd12b253e
RESEARCH PRODUCT
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subject
animal structuresendocrine system diseasesGeneral Physics and AstronomySubventricular zoneBiologyGeneral Biochemistry Genetics and Molecular BiologySubgranular zone03 medical and health sciencesParacrine signalling0302 clinical medicinemedicineAutocrine signalling030304 developmental biology0303 health sciencesMultidisciplinaryNeurogenesisGeneral ChemistryMolecular biologyfemale genital diseases and pregnancy complicationsNeural stem cellCell biologyOlfactory bulbmedicine.anatomical_structurenervous systemGenomic imprinting030217 neurology & neurosurgerydescription
AbstractGenomic imprinting is implicated in the control of gene dosage in neurogenic niches. Here we address the importance of Igf2 imprinting for murine adult neurogenesis in the subventricular zone (SVZ) and in the subgranular zone (SGZ) of the hippocampus in vivo. In the SVZ, paracrine IGF2 is a cerebrospinal fluid and endothelial-derived neurogenic factor requiring biallelic expression, with mutants having reduced activation of the stem cell pool and impaired olfactory bulb neurogenesis. In contrast, Igf2 is imprinted in the hippocampus acting as an autocrine factor expressed in neural stem cells (NSCs) solely from the paternal allele. Conditional mutagenesis of Igf2 in blood vessels confirms that endothelial-derived IGF2 contributes to NSC maintenance in SVZ but not in the SGZ, and that this is regulated by the biallelic expression of IGF2 in the vascular compartment. Our findings indicate that a regulatory decision to imprint or not is a functionally important mechanism of transcriptional dosage control in adult neurogenesis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-09-15 | Nature Communications |